Engineering a Plant Activation Tagging method using transposons

Strategies that aid the discovery of new gene functions in plants are important tools that can help to improve traits to cope with the challenges that agriculture and plant-based industries face nowadays. They are also useful from a scientific point of view, contributing to the knowledge about the genetic regulation of plant processes in general. In this work, a transposon-based strategy to obtain gain-of-function mutants in the model plant Arabidopsis thaliana is presented. This strategy allows the discovery of new gene functions that can be difficult to find with other, such as loss-of-function, strategies.